CSK Antibody (YA2742)

CSK Antibody (YA2742) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CSK.

CSK Protein, a non-receptor tyrosine-protein kinase, holds a pivotal role in the intricate regulation of cell growth, differentiation, migration, and immune response. It accomplishes this by phosphorylating tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) such as LCK, SRC, HCK, FYN, LYN, CSK, or YES1. This phosphorylation event induces intramolecular interactions between the phosphotyrosine tail and the SH2 domain of SFKs, resulting in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane through binding to transmembrane proteins or adapter proteins in close proximity. CSK plays a crucial role in suppressing signaling by various surface receptors, including the T-cell receptor (TCR) and B-cell receptor (BCR), by phosphorylating and maintaining several positive effectors such as FYN or LCK in an inactive state, thereby modulating key cellular responses.

P41240

Predicted band size: 51 kDa; Observed band size: 51 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3105115

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100

• 
  Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using CSK Antibody (YA2742). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82997, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using CSK Antibody (YA2742). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82997, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P

Liquid

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human CSK