CREBBP Antibody (YA5492)

CREBBP Antibody (YA5492) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CREBBP.

CREBBP is an acetyltransferase that acetylates histones (e.g., H3K18ac and H3K27ac), providing specific marks for transcriptional activation. It also acetylates non-histone proteins, including DDX21, FBL, IRF2, MAFG, NCOA3, POLR1E/PAF53, and FOXO1. CREBBP specifically binds phosphorylated CREB, enhancing its transcriptional activity toward cAMP-responsive genes, and acts as a coactivator for ALX1. It functions as a circadian transcriptional coactivator by enhancing the activity of NPAS2-BMAL1 and CLOCK-BMAL1 heterodimers. During nucleotide excision repair (NER), CREBBP acetylates PCNA, promoting its removal from chromatin and degradation. Additionally, it acetylates POLR1E/PAF53, reducing RNA polymerase I association with the rDNA promoter and coding regions. CREBBP inhibits DDX21 helicase activity via acetylation and prevents histone H2A Gln-105 methylation (H2AQ104me) by acetylating FBL. Beyond protein acetylation, CREBBP utilizes different acyl-CoA substrates (e.g., lactoyl-CoA) to mediate protein lactylation, such as MRE11 lactylation in response to DNA damage, promoting homologous recombination (HR)-mediated DNA double-strand break (DSB) repair. It also serves as a transcriptional coactivator for SMAD4 in the TGF-beta signaling pathway.

Q92793

Predicted band size: 266 kDa; Observed band size: 266 kDa

Protein G

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-2000; ICC/IF: 1:100-500; ELISA: 1:1000-5000

WB, ICC/IF, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Synthesized peptide derived from human CREBBP AA range: 900-1000