CREB Antibody (YA491)

CREB Antibody (YA491) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CREB.

Creb is a phosphorylation-dependent transcription factor that stimulates transcription by binding to the cAMP response element (CRE) in DNA, a sequence found in many viral and cellular promoters (By similarity). Its transcriptional activation is enhanced by TORC coactivators, which function independently of Ser-119 phosphorylation (PubMed:14536081). Creb is involved in various cellular processes, including the synchronization of circadian rhythms and the differentiation of adipose cells (By similarity). Additionally, it regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling (By similarity).

Free

P16220

Predicted band size: 35 kDa；Observed band size: 42 kDa

Protein A affinity purified.

Nucleus

Non-conjugated

Unmodified

AB_3102299

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat, Zebrafish

WB: 1:500 ; ICC/IF: 1:50 ; IHC-P: 1:50-1:500 ; FC: 1:50 ; ICC/IF: 1:500

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  Western blot analysis of extracts from NIH3T3 (lane 1) and MDA-MB-231 (lane 2) and Hela (lane 3) and Jurkat (lane 4) and M-lymph (lane 5) and K562 (lane 6) using CREB antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, IHC-P, ICC/IF, FC, IF-Tissue

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human CREB.AA range:250-290.