Collagen I Antibody

Cat. No.: HY-P81227: Data Sheet COA
SDS
User Guide for Antibodies
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Collagen I Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Collagen I.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Collagen I Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Collagen I.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 139 kDa;

Observed band size: 139 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Rabbit Predicted Reactivity: Chicken, Dog, Cow, Sheep

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

P02452

Gene ID

1277 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from human Collagen I: 1051-1150/1464

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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ALL WB IHC-P

Western blot analysis of extracts from K562 using Collagen I antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

RRID

AB_3103110

Database

Entrez Gene: 1277 Human ; 1278 Human ; 12842 Mouse ; 12843 Mouse ; 29393 Rat 84352

SwissProt: P02452 Human ; P08123 Human ; P11087 Mouse ; Q01149 Mouse ; P02454 Rat P02466

OMIM: 114000 Human

Synonyms

Collagen type I; Alpha 1 type I collagen; Alpha 2 type I collagen; COL1A1; COL1A2; Collagen I alpha 1 polypeptide; Collagen I alpha 2 polypeptide; Collagen Of Skin Tendon And Bone; Collagen Type 1; Collagen type I alpha 1; Collagen type I alpha 2; OI4; Osteogenesis Imperfecta Type IV; Pro alpha 1(I) collagen; Type I procollagen; CO1A1_HUMAN.

Documentation

Select Batch:

Data Sheet (231 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Collagen I Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MedchemExpress Validation
Western blot analysis of extracts from K562 using Collagen I antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
MedchemExpress Validation
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
MedchemExpress Validation
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Collagen I Antibody purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2025 Aug 26:220:107933. [Abstract]

Customer Validation
IHC staining of fibrosis markers α-SMA, Collagen I, Vimentin and E-cadherin (n = 3). Scale bar: 100 μm.

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Collagen I Antibody

Collagen I Antibody Featured Recommendations:

Publications Citing Use of MCE Collagen I Antibody

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Documentation

Collagen I Antibody Related Classifications

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