Collagen I Antibody

Cat. No.: HY-P81227: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Collagen I Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Collagen I.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

Collagen I Antibody Featured Recommendations:

Publications Citing Use of MCE Collagen I Antibody

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

Biological Activity
Technical Parameters
Properties
Documentation

Description	Collagen I Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Collagen I.
Background	Collagen I is a member of group I collagen (fibrillar forming collagen).
Gene ID	1277 [NCBI]
SwissProt ID	P02452
Synonyms	Collagen type I; Alpha 1 type I collagen; Alpha 2 type I collagen; COL1A1; COL1A2; Collagen I alpha 1 polypeptide; Collagen I alpha 2 polypeptide; Collagen Of Skin Tendon And Bone; Collagen Type 1; Collagen type I alpha 1; Collagen type I alpha 2; OI4; Osteogenesis Imperfecta Type IV; Pro alpha 1(I) collagen; Type I procollagen; CO1A1_HUMAN.
Molecular Weight	Predicted band size: 139 kDa; Observed band size: 139 kDa
Purity	affinity purified
Subcellular Location	Secreted, extracellular space, extracellular matrix.
Conjugation	Non-conjugated
Modification	Unmodified
RRID	AB_3103110
Product Categories	Primary Antibody; Rabbit Polyclonal Antibody
Clonality	Polyclonal
Host	Rabbit
Reactivity	Human, Mouse, Rat, Rabbit
Predicted Reactivity	Chicken, Dog, Cow, Sheep Note: The predicted reactivity is for reference only, not as a product performance guarantee.
Dilution Ratio	WB: 1:500-2000; IHC-P: 1:100-500 Western blot analysis of extracts from K562 using Collagen I antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature. Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application	WB, IHC-P
Appearance	Liquid
Formulation	Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Sensitivity	Endogenous
Immunogen	KLH conjugated synthetic peptide derived from human Collagen I: 1051-1150/1464
Database	Entrez Gene: 1277 Human ; 1278 Human ; 12842 Mouse ; 12843 Mouse ; 29393 Rat 84352 SwissProt: P02452 Human ; P08123 Human ; P11087 Mouse ; Q01149 Mouse ; P02454 Rat P02466 OMIM: 114000 Human
Documentation	Select Batch: Data Sheet (231 KB) SDS (252 KB) COA (205 KB) User Guide for Antibodies (1077 KB)

Collagen I Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Please select a local distributor ナミキ商事株式会社コスモ・バイオ株式会社重松貿易株式会社
Product Name			Requested Quantity *
Applicant Name *			Salutation
Email Address *			Phone Number *
Department			Organization Name *
City			State
Country or Region *
Remarks

Bulk Inquiry

Inquiry Information

Product Name:: Collagen I Antibody
Cat. No.:: HY-P81227
Quantity:
MCE Japan Authorized Agent:

Western blot analysis of extracts from K562 using Collagen I antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western blot analysis of extracts from K562 using Collagen I antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Collagen I Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81227, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Name			Lot #
Applicant Name *			Country or Region *
Email Address *
Phone Number *			Organization Name *
Remarks

MedChemExpress: Contact Us; About Us; Headquarters; Distributors; Statement on False Report; Careers

Customer Support: Technical Support; Service; Ordering Information; Easy Return; Shipping Rates & Policies; Intellectual Property Promise

Resources: Free Sample; Resources; Molarity Calculator; Dilution Calculator; Terms & Conditions; Reconstitution Calculator; Specific Activity Calculator; DNA/RNA sequence conversion tools

Subscribe to our E-newsletter

Name
Email *

	Sorry, but the email address you supplied was invalid.

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.

Join with us

Collagen I Antibody

Collagen I Antibody Featured Recommendations:

Publications Citing Use of MCE Collagen I Antibody

Biological Activity

Technical Parameters

Properties

Documentation

Collagen I Antibody Related Classifications

Your Recently Viewed Products:

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report