Cofilin Antibody (YA2822)

Cofilin Antibody (YA2822) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2822), targeting Cofilin, with a predicted molecular weight of 19 kDa (observed band size: 19 kDa). Cofilin Antibody (YA2822) can be used for WB, IHC-F, IHC-P, ICC/IF experiment in human background.

Cofilin binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. In conjunction with the subcortical maternal complex (SCMC), it plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. It is required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Cofilin plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells. It is also required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Additionally, Cofilin is involved in neural tube morphogenesis and neural crest cell migration (By similarity).

P23528

Predicted band size: 19 kDa; Observed band size: 19 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3105193

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200

• 
  Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), and C6 (lane 2, 20 μg) using Cofilin Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-F, IHC-P, ICC/IF

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human Cofilin