Clusterin alpha Chain Antibody (YA2525)

Clusterin alpha Chain Antibody (YA2525) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2525), targeting Clusterin alpha Chain, with a predicted molecular weight of 52 kDa (observed band size: 42 kDa). Clusterin alpha Chain Antibody (YA2525) can be used for WB, IP experiment in rat background.

Clusterin alpha Chain functions as an extracellular chaperone that prevents aggregation of non-native proteins, including stress-induced aggregation of plasma proteins (By similarity). It inhibits amyloid fibril formation by APP, APOC2, B2M, CALCA, CSN3, SNCA, and aggregation-prone LYZ variants in vitro (PubMed:14741101). ATP-independent, it maintains partially unfolded proteins for refolding by other chaperones like HSPA8/HSC70 but lacks autonomous refolding activity. Binding to cell surface receptors triggers internalization and lysosomal/proteasomal degradation of client complexes. Secreted forms protect against apoptosis and complement-mediated cytolysis by blocking C9 polymerization in the MAC (By similarity). Intracellularly, it interacts with ubiquitin and SCF E3 ligases to promote degradation of targets (e.g., COMMD1, IKBKB) and modulates NF-kappa-B activity (By similarity). Under stress, it can either promote apoptosis (PubMed:12551933) or inhibit it via mitochondrial BAX-cytochrome c interference. It regulates proliferation and, during ER stress, stabilizes mitochondrial membranes via HSPA5 binding (By similarity). Secreted forms do not affect caspase/BAX-mediated apoptosis or TNF-induced NF-kappa-B (By similarity) but modulate neuronal differentiation through STMN3 interaction (By similarity) and clear injury-related immune complexes (PubMed:11865066).

Q06890

Predicted band size: 52 kDa; Observed band size: 42 kDa

Affinity Purified

Non-conjugated

Unmodified

Immunology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Rat

WB: 1/500-1/1000;IP: 1/20

WB, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Recombinant protein of mouse Clusterin alpha chain