Clathrin Heavy Chain Antibody (YA501)

Clathrin Heavy Chain Antibody (YA501) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Clathrin Heavy Chain.

Clathrin heavy chain is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. It acts as a component of the TACC3/ch-TOG/clathrin complex, which contributes to the stabilization of kinetochore fibers of the mitotic spindle by acting as an inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. It plays a role in early autophagosome formation. Interaction with DNAJC6 mediates the recruitment of HSPA8 to the clathrin lattice and creates local destabilization of the lattice, promoting uncoating (by similar).

Free

Q00610

Predicted band size: 180 kDa; Observed band size: 180 kDa

Protein A affinity purified.

Cytoplasmic vesicle membrane.

Non-conjugated

Unmodified

AB_3102297

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) ) , HUVEC (lane 3(20μg) and NIH3T3 (lane 4(20μg) ) using Clathrin heavy chain Rabbit mAb（HY-P80086）. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin , HY-P80438, 1/10000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Clathrin heavy chain Antibody. The section was pre-treated using heat mediated antigen retrieval with Trsi-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80086, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Clathrin heavy chain Antibody. The section was pre-treated using heat mediated antigen retrieval with Trsi-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80086, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Clathrin heavy chain.AA range:201-250.