CHK2 Antibody (YA3581)

CHK2 Antibody (YA3581) is a mouse-derived and non-conjugated IgG2b antibody, targeting to CHK2. It can be applicated for WB, IHC-P, ICC/IF, ELISA assays, in the background of human.

Chk2 is a serine/threonine-protein kinase essential for checkpoint-mediated cell cycle arrest, DNA repair activation, and apoptosis in response to DNA double-strand breaks. It phosphorylates CDC25A/B/C to inhibit their activity, blocking cell cycle progression, and enhances homologous recombination repair by phosphorylating BRCA2 to promote RAD51-chromatin association. Chk2 regulates apoptosis via phosphorylation of p53/TP53, MDM4, and PML, while activating DNA repair and pro-apoptotic gene transcription through FOXM1 and E2F1. Its tumor suppressor role extends to mitotic spindle assembly via BRCA1 phosphorylation. Under oxidative stress, Chk2 phosphorylates TRIM32 to ubiquitinate ATG7, promoting autophagosome assembly (by similar: during microbial infection, it phosphorylates HHV-1 ICP0 to activate its SUMO-targeted ubiquitin ligase activity).

O96017

Predicted band size: 61 kDa; Observed band size: 61 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

WB: 1/500-1/2000; IHC: 1/200-1/1000; IF: 1/200-1/1000; ELISA: 1/10000

WB, IHC-P, ICC/IF, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Purified recombinant fragment of human CHK2 (aa481-531) expressed in E. Coli.