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  5. CDK8 Antibody (YA1323)

CDK8 Antibody (YA1323)

Cat. No.: HY-P81578
User Guide for Antibodies Technical Support

CDK8 Antibody (YA1323) is a non-conjugated IgG antibody, targeting CDK8, with a predicted molecular weight of 53 kDa (observed band size: 55 kDa). CDK8 Antibody (YA1323) can be used for IHC-P experiment in human, mouse background.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

CDK8 Antibody (YA1323) is a non-conjugated IgG antibody, targeting CDK8, with a predicted molecular weight of 53 kDa (observed band size: 55 kDa). CDK8 Antibody (YA1323) can be used for IHC-P experiment in human, mouse background.
Background

The CDK8-CCNC-MED12 protein is a crucial component of the Mediator complex, a coactivator integral to the regulation of gene transcription for nearly all RNA polymerase II-dependent genes. As part of the Mediator complex, it acts as a bridge, conveying information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. This complex is recruited to promoters through direct interactions with regulatory proteins, serving as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and general transcription factors. CDK8-CCNC-MED12 exerts its regulatory functions by phosphorylating the C-terminal domain (CTD) of the large subunit of RNA polymerase II, potentially inhibiting the formation of a transcription initiation complex. Furthermore, it phosphorylates CCNH, leading to the down-regulation of the TFIIH complex and subsequent transcriptional repression. Notably, CDK8-CCNC-MED12 is recruited through interaction with MAML1, contributing to the hyperphosphorylation of the intracellular domain of NOTCH and facilitating its degradation. These multifaceted roles underscore the significance of CDK8-CCNC-MED12 in orchestrating the intricate processes of gene transcription regulation.
Gene ID
SwissProt ID
Synonyms
Cyclin-dependent kinase 8
Molecular Weight

Predicted band size: 53 kDa; Observed band size:55 kDa
Purity

Protein A
Conjugation

Non-conjugated
Modification

Unmodified
Research Field

Cell biology
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse
Dilution Ratio

IHC-P：1/50-1/100
Application

IHC-P
Appearance

Liquid
Formulation

Supplied in PBS, Glycerol and BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

Peptide
Database

Entrez Gene: 1024 Human ; 264064 Mouse ;

SwissProt: P49336 Human ; Q8R3L8 Mouse ;

OMIM: 618748 Human

Documentation
User Guide for Antibodies (1077 KB)

CDK8 Antibody (YA1323) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CDK8 Antibody (YA1323)
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HY-P81578
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