CDK2 Antibody (YA3903)

CDK2 Antibody (YA3903) is a mouse-derived and non-conjugated IgG1 antibody, targeting to CDK2. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse.

CDK2, a serine/threonine-protein kinase, intricately regulates the cell cycle, playing an indispensable role in meiosis while being dispensable for mitosis. Operating at the nexus of cellular processes, CDK2 phosphorylates a diverse array of substrates, including CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, and EZH2. It orchestrates critical events such as centrosome duplication and DNA synthesis initiation at the G1-S transition, modulating G2 progression and finely tuning the balance between cellular proliferation, death, and DNA repair in human embryonic stem cells. Activated sequentially by cyclin E and cyclin A2, CDK2 regulates the transition from S phase to mitosis. Notably, its phosphorylation of EZH2 is implicated in maintaining H3K27me3 and epigenetic gene silencing. CDK2's involvement extends to DNA damage responses, where it regulates the G1-S phase checkpoint, phosphorylates BRCA2, and modulates homologous recombination-dependent repair. Furthermore, CDK2 participates in diverse signaling pathways, including vitamin D-mediated growth inhibition, NO-mediated signaling, and the phosphorylation of substrates such as FOXP3 and ERCC6, highlighting its multifaceted role in cellular physiology.

P24941

Predicted band size: 34 kDa; Observed band size: 34 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse

WB: 1/500-1/2000; IHC: 1/200-1/1000; IF: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human CDK2 expressed in E. Coli.