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  5. CDC40 Antibody (YA3168)

CDC40 Antibody (YA3168)

Cat. No.: HY-P83423
COA
SDS
User Guide for Antibodies Technical Support

CDC40 Antibody (YA3168) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDC40.

For research use only. We do not sell to patients.

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10 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  
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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

CDC40 Antibody (YA3168) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDC40.
Background

CDC40 is essential for pre-mRNA splicing as a component of the activated spliceosome (by similar: PubMed:33220177). It plays a critical role in embryonic brain development, independent of proline isomerization activity (by similar: PubMed:33220177).
Gene ID
SwissProt ID
Synonyms
cell division cycle 40; EH-binding protein 3; EHB3; pre-mRNA splicing factor 17; PRP17; PRPF17; Cdc40
Molecular Weight

Predicted band size: 66 kDa; Observed band size: 74 kDa
Purity

Affinity Chromatography
Conjugation

Non-conjugated
Modification

Unmodified
Research Field

Epigenetics and Nuclear Signaling
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse, Rat
Dilution Ratio

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; FC: 1:50-1:100

  • Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), K562 (lane 2, 15 μg), 293T (lane 3, 15 μg), and HUVEC (lane 4, 15 μg) using CDC40 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Application

WB, IHC-P, ICC/IF, FC
Appearance

Liquid
Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

A synthesized peptide derived from human CDC40
Database

Entrez Gene: 51362 Human ; 71713 Mouse ; 361859 Rat

SwissProt: O60508 Human ; Q9DC48 Mouse ;

OMIM: 619302 Human

Documentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

CDC40 Antibody (YA3168) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CDC40 Antibody (YA3168)
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HY-P83423
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