CD80 Antibody (YA3386)

CD80 Antibody (YA3386) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to CD80.

B7-1 is a costimulatory molecule belonging to the immunoglobulin superfamily and plays a crucial role in T-lymphocyte activation. It acts as the primary auxiliary signal, augmenting the MHC/TCR signal in naive T-cells alongside the constitutively expressed CD28 receptor. This interaction activates signaling pathways such as NF-kappa-B and MAPK, leading to cytokine production. Additionally, the CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis in T-cells via the PI3K/Akt pathway. B7-1 also regulates PDL1/PDCD1 interactions to limit excessive PDL1 engagement and its immunosuppressive effects. Expressed on B-cells, it is critical for modulating B-cell and T-cell interactions during germinal center responses, essential for effective humoral immunity (By similarity). Furthermore, B7-1 serves as a receptor for adenovirus subgroup B (Microbial infection).

P33681

Predicted band size: 32 kDa,Observed band size: 48 kDa

affinity purified

Membrane; Single-pass type I membrane protein.

Non-conjugated

Unmodified

AB_3103091

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse

Rat

WB: 1:500-2000; IHC-P: 1:100-500; FC: 1ug:Test;ICC: 1:50;

• 
  Western blot analysis of extracts from RAW264.7 using CD80 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-mouse IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, IHC-P, FC, ICC/IF

Liquid

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Recombinant mouse CD80 protein: 38-246/306