CD8 alpha Antibody (YA6308)

CD8 alpha Antibody (YA6308) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD8 α. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

The CD8A, an integral membrane glycoprotein, plays a critical role in the immune response, fulfilling multiple functions in responses against both external and internal threats. In T-cells, it primarily functions as a coreceptor for the MHC class I molecule:peptide complex, interacting simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen-presenting cells (APCs). This interaction leads to the recruitment of the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK, in turn, initiates diverse intracellular signaling pathways, phosphorylating various substrates and ultimately promoting lymphokine production, motility, adhesion, and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing the conjugation and lysis of multiple target cells. CD8A homodimer molecules also contribute to the survival and differentiation of activated lymphocytes into memory CD8 T-cells. The CD8A forms disulfide-linked complexes at the cell surface and also homodimers in various cell types, including NK-cells and peripheral blood T-lymphocytes. It interacts with the MHC class I HLA-A/B2M dimer and with LCK in a zinc-dependent manner.

P01732

Predicted band size: 26 kDa; Observed band size: 35 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:200-1000; WB: 1:1000-5000; ICC/IF: 1:200-1000; ELISA: 1:5000-20000; IP: 1:50-200

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG