CD5 Antibody (YA1404)

CD5 Antibody (YA1404) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD5.

The CD5 protein is distinguished by its deficiency in conserved residue(s) crucial for the propagation of feature annotation.

P06127

Predicted band size: 55 kDa; Observed band size: 55 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3103768

Immunology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal, Recombinant

Rabbit

Human

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; IP: 1:50

• 
  Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg) using CD5 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P, ICC/IF, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human CD5.