CD45 Antibody (YA533)

CD45 Antibody (YA533) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD45.

Receptor-type tyrosine-protein phosphatase C (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family, also known as CD45, is a transmembrane glycoprotein. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation.
PTPRC contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP.
PTPRC has been shown to be an essential regulator of T- and B-cell antigen receptor signaling as PTPRC positive regulate T-cell coactivation upon binding to DPP4, recruiting and dephosphorylating SKAP1 and FYN. PTPRC also dephosphorylates LYN, and thereby modulates LYN activity.
PTPRC functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. PTPRC also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling.
PTPRC gene has many alternatively spliced transcripts variants, which encode distinct isoforms.

Free

P08575

Predicted band size: 147 kDa；Observed band size: 200 kDa

Protein A affinity purified.

Cell junction, Cell membrane, Membrane

Non-conjugated

Unmodified

AB_3102064

Immunology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

• 
  Western blot analysis of extracts from Jurkat(lane 2(20μg) and Jurkat(lane 3(40μg) using CD45 (HY-P80063 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IHC-P, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human CD45.AA range:1210-1306.