PD-L1 Antibody (YA4539)

CD274 Antibody (YA4539) is a mouse-derived and non-conjugated IgG2b antibody, targeting to CD274. It can be applicated for IHC-P, FC, ELISA assays, in the background of human.

CD274 plays a critical role in the induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, it modulates the activation threshold of T-cells and limits T-cell effector responses. Through an unknown activating receptor, CD274 may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). It can also act as a transcription coactivator: in response to hypoxia, it translocates into the nucleus via interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape immune system destruction, thereby facilitating tumor survival. The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (by similar). Blocking the PDCD1-mediated pathway reverses the exhausted T-cell phenotype and normalizes the anti-tumor response, providing a rationale for cancer immunotherapy (by similar).

Q9NZQ7

Predicted band size: 33 kDa; Observed band size: 46 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

IHC: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

IHC-P, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Purified recombinant fragment of human CD274 (AA: 24-153) expressed in E. Coli.