CD223/LAG-3 Antibody (YA3842)

CD223/LAG-3 Antibody (YA3842) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD223/LAG-3.

CD223 (Lymphocyte activation gene 3 protein) is an inhibitory receptor primarily expressed on antigen-activated T-cells. It delivers inhibitory signals upon binding to ligands such as FGL1 (By similarity). FGL1 serves as a major ligand for LAG3 and mediates its T-cell inhibitory function (By similarity). Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse, directly suppressing T-cell activation (By similarity). It may synergize with PDCD1/PD-1 to inhibit antigen-specific T-cell activation, potentially acting as a coreceptor for PDCD1/PD-1 (By similarity). LAG3 negatively regulates the proliferation, activation, effector function, and homeostasis of both CD8(+) and CD4(+) T-cells. Additionally, it mediates immune tolerance by being constitutively expressed on a subset of regulatory T-cells (Tregs) and contributing to their suppressive function (By similarity). It also acts as a negative regulator of plasmacytoid dendritic cell (pDC) activation (By similarity). While LAG3 binds MHC class II (MHC-II), its precise role remains unclear. It may function as a ligand for MHC-II on antigen-presenting cells (APCs), promoting their activation/maturation and driving Th1 immune responses.

P18627 

Predicted band size: 57 kDa; Observed band size: 95 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal Antibody

Mouse

Human

WB: 1:500-1:2000; FC: 1:200-1:400; ELISA: 1:10000

WB, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human CD223 (AA: extra 66-199) expressed in E. Coli.