CD16 Antibody

Cat. No.: HY-P81202: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

CD16 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CD16.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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CD16 Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

CD16 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CD16.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 27kDa;

Observed band size: 50-65 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat Predicted Reactivity: Pig, Cow, Rabbit, Sheep

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

P08637

Gene ID

2214 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from human IGFR3/CD16: 131-230/254

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-10000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
FC FC: Flow Cytometry	1ug:Test
IF	1:100-500

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from M-Lung (lane 1) and M-Liver (lane 2) using CD16 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

RRID

AB_3103098

Database

Entrez Gene: 2214 Human ;

SwissProt: P08637 Human ; P08508 Mouse ;

OMIM: 615707 Human

Synonyms

FCG3A_HUMAN; Low affinity immunoglobulin gamma Fc region receptor III-A; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; CD_antigen: D16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3.

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

CD16 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CD16 Antibody

CD16 Antibody Featured Recommendations:

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Background

Documentation

CD16 Antibody Related Classifications

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