LAMP2 Antibody (YA3778)

LAMP2 Antibody (YA3778) is a mouse-derived and non-conjugated IgG2b antibody, targeting to CD107b. It can be applicated for WB, IHC-P, FC, ELISA assays, in the background of human, mouse, rat.

CD107b is a lysosomal membrane glycoprotein that plays a crucial role in lysosome biogenesis, lysosomal pH regulation, and autophagy. It acts as a key regulator of lysosomal lumen pH by directly inhibiting the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity. CD107b is essential for chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins under stress conditions and during the normal turnover of long-lived proteins. It functions by binding target proteins such as GAPDH, NLRP3, and MLLT11, directing them for lysosomal degradation. In chaperone-mediated autophagy, CD107b operates downstream of chaperones like HSPA8/HSC70, which recognize and recruit substrate proteins to lysosomes, where they bind to LAMP2. Additionally, CD107b is involved in lysosomal protein degradation during starvation (by similarity) and mediates the fusion of autophagosomes with lysosomes during autophagy. Cells lacking LAMP2 fail to accumulate STX17 on autophagosomes, likely explaining the impaired fusion process. CD107b also contributes to MHC class II-mediated presentation of exogenous antigens by facilitating lysosomal protein degradation, generating antigenic peptides captured by MHC II subunits. However, it is not required for the presentation of endogenous antigens. Furthermore, CD107b supports FURIN-mediated cleavage of the mumps virus fusion protein F by interacting with both FURIN and the unprocessed form of the viral protein F.

P13473

Predicted band size: 45 kDa; Observed band size: 110 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse, Rat

WB: 1/500-1/2000; IHC: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Purified recombinant fragment of human CD107b (AA: extra 29-168) expressed in E. Coli.