CBX4 Antibody (YA3487)

CBX4 Antibody (YA3487) is a mouse-derived and non-conjugated IgG1 antibody, targeting to CBX4. It can be applicated for IHC-P, FC, ELISA assays, in the background of human.

CBX4 is an E3 SUMO-protein ligase that catalyzes the sumoylation of target proteins by facilitating the transfer of SUMO from the E2 enzyme to the substrate. It is involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, thereby indirectly regulating p53/TP53 transcriptional activation and leading to p21/CDKN1A expression. Additionally, CBX4 monosumoylates ZNF131. CBX4 is a component of the Polycomb group (PcG) multiprotein PRC1-like complex, which is essential for maintaining the transcriptionally repressive state of numerous genes, including Hox genes, during development. The PcG PRC1 complex functions through chromatin remodeling and histone modifications, mediating monoubiquitination of histone H2A at 'Lys-119', resulting in heritable changes in chromatin expressibility. CBX4 also binds to histone H3 trimethylated at 'Lys-9' (H3K9me3) (by similar). It plays a role in germ layer lineage differentiation during embryonic development (by similar).

O00257

Predicted band size: 61 kDa; Observed band size: 61 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

IHC: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

IHC-P, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human CBX4 (AA: 397-514) expressed in E. Coli.