CBR1 Antibody (YA810)

CBR1 Antibody (YA810) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CBR1.

CBR1 (2C9): The protein encoded by this gene belongs to the short-chain dehydrogenases/reductases (SDR) family, which function as NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds, such as quinones, prostaglandins, and various xenobiotics. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2013]

Free

P16152

Predicted band size: 30 kDa; Observed band size: 30 kDa

affinity purified

Non-conjugated

Unmodified

Signal Transduction

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:1,000 ;IF: 1:50-1:200

• 
  Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), SH-SY5Y (lane 3, 15 μg), A549 (lane 4, 15 μg), MCF-7 (lane 5, 15 μg), and SK-OV-3 (lane 6, 15 μg) using CBR1 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, ICC/IF

Liquid

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Endogenous

Synthetic peptide corresponding to Human CBR1.The exact sequence is proprietary to MCE.