CBFB Antibody (YA2006)

CBFB Antibody (YA2006) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CBFB.

CBFB, a core-binding factor, forms the heterodimeric complex, CBF, with RUNX family proteins, including RUNX1, RUNX2, and RUNX3. RUNX members exert transcriptional modulation on their target genes by recognizing the core consensus binding sequence 5'-TGTGGT-3', or exceptionally, 5'-TGCGGT-3', within regulatory regions through their runt domain. CBFB serves as a non-DNA-binding regulatory subunit that enhances the sequence-specific DNA-binding capacity of RUNX allosterically. These heterodimers bind to the core sites of various enhancers and promoters, such as murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3, and GM-CSF promoters. CBF complexes play a role in the repression of the ZBTB7B transcription factor during cytotoxic (CD8+) T cell development, acting as a transcriptional silencer by binding to the RUNX-binding sequence within the ZBTB7B locus, thereby facilitating cytotoxic T cell differentiation. The interaction involves heterodimerization with RUNX1, RUNX2, and RUNX3, and CBFB also interacts with COPRS and forms a complex with PRMT5 and RUNX1.

Q13951

Predicted band size: 22 kDa; Observed band size: 22 kDa

Affinity Chromatography

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; FC: 1:50-1:100

• 
  Western blot analysis was performed on extracts from Raji (lane 1, 15 μg), K562 (lane 2, 15 μg), Jurkat (lane 3, 15 μg), and RAW264.7 (lane 4, 15 μg) using CBFB Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:30000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P, ICC/IF, FC

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human CBFb