Caspase-1 Antibody

Caspase-1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Caspase-1.

Caspase-1 is a thiol protease involved in various inflammatory processes by proteolytically cleaving other proteins, such as the precursors of inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18), as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides. As an inflammatory response initiator, Caspase-1 plays a key role in cell immunity: once activated through inflammasome complex formation, it triggers a pro-inflammatory response by cleaving IL1B and IL18, releasing mature cytokines involved in inflammatory processes. It cleaves a tetrapeptide after an Asp residue at position P1. Additionally, Caspase-1 initiates pyroptosis, a programmed lytic cell death pathway, by cleaving GSDMD. Unlike IL1B cleavage, GSDMD recognition and cleavage depend on an exosite interface on Caspase-1 that binds the C-terminal part of Gasdermin-D (GSDMD-CT). During bacterial infection, Caspase-1 cleaves and activates CASP7, promoting plasma membrane repair. Upon inflammasome activation in DNA virus infections (but not RNA viruses), it controls antiviral immunity by cleaving CGAS, inactivating it. In apoptotic cells, Caspase-1 cleaves SPHK2, which is released and remains enzymatically active extracellularly (by similar).

P29452

Predicted band size: 46 kDa; Observed band size: 46 kDa

affinity purified

Cytoplasm.

Non-conjugated

Unmodified

AB_3644161

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat,

WB: 1:500-2000; ELISA: 1:5000-10000; IHC-P: 1:100-500; IHC-F: 1:100-500; FC: 1μg/Test;ICC: 1:100-500; IF: 1:100-500

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  Western blot analysis of extracts from M-spleen (lane 1) and 293T (lane 2) using Caspase-1 p20 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ELISA, IHC-P, IHC-F, FC, ICC/IF

Liquid

Supplied in 1% BSA, 0.01M PBS, 15mM Sodium Azide pH 7.4

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from mouse Caspase-1 p20: 181-280/402