CASP4 Antibody

CASP4 Antibody is a non-conjugated IgG antibody, targeting CASP4, with a predicted molecular weight of 43 kDa. CASP4 Antibody can be used for WB, ELISA experiment in human and rat background.

CASP4 is an inflammatory caspase that functions as the effector of the non-canonical inflammasome by mediating lipopolysaccharide (LPS)-induced pyroptosis. It also indirectly activates the NLRP3 and NLRP6 inflammasomes and acts as a thiol protease, cleaving a tetrapeptide after an Asp residue at position P1 to catalyze the cleavage of CGAS, GSDMD, and IL18. Within the non-canonical inflammasome, CASP4 is activated by direct binding to the lipid A moiety of LPS without requiring an upstream sensor, leading to the cleavage of GSDMD and IL18, followed by IL18 secretion through the GSDMD pore, pyroptosis of infected cells, and their extrusion into the gut lumen. Additionally, it indirectly promotes the secretion of mature cytokines (IL1A and HMGB1) downstream of GSDMD-mediated pyroptosis via activation of the NLRP3 and NLRP6 inflammasomes. CASP4 plays a crucial role in restricting S.typhimurium replication in colonic epithelial cells and is involved in cell death induced by endoplasmic reticulum stress and cytotoxic APP peptides found in Alzheimer's patient brains. Notably, GSDMD cleavage is not strictly dependent on the consensus cleavage site but relies on an exosite interface on CASP4 that recognizes and binds the Gasdermin-D C-terminal (GSDMD-CT) portion. By similarity, it also acts as an activator of adaptive immunity in dendritic cells in response to oxidized phospholipid oxPAPC, and excessive activation of the non-canonical inflammasome in brain endothelial cells may lead to blood-brain barrier breakdown.

P49662

Predicted band size: 43 kDa;Observed band size: 54 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3103120

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Rat

Mouse

WB: 1:500-2000;ELISA: 1:5000-10000;

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  Western blot analysis of extracts from THP-1 (lane 1) and Raji (lane 2) and Hela (lane 3) and HepG2 (lane 4) using CASP4 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ELISA

Liquid

Supplied in 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from mouse Caspase-4 subunit p10: 251-350/373