CARM1 Antibody (YA5184)

CARM1 Antibody (YA5184) is a mouse-derived and non-conjugated monoclonal antibody, targeting to CARM1. It can be applicated for WB, IP assays, in the background of human, mouse.

CARM1 is a methyltransferase that mono- and asymmetrically dimethylates arginine residues in proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. It collaborates with EP300/P300 and p160 family histone acetyltransferases to methylate histone H3 at Arg-17 (H3R17me, primarily forming H3R17me2a), activating transcription via chromatin remodeling. During nuclear hormone receptor or TCF7L2/TCF4 activation, CARM1 synergizes with EP300/P300 and p160 acetyltransferases (e.g., NCOA1/SRC1, NCOA2/GRIP1, NCOA3/ACTR) or CTNNB1/beta-catenin to enhance transcription (by similar). In myogenic activation, it acts as a coactivator for MEF2C with NCOA3/ACTR (by similar). It also participates in monocyte inflammatory responses, PPARG-mediated adipocyte differentiation, pre-mRNA splicing regulation, and p53/TP53 transcriptional activation (by similar). CARM1 methylates EP300/P300 at specific arginine sites, disrupting its interaction with CREB, and modifies RNA-binding proteins (e.g., PABPC1, ELAVL1, ELAV4), potentially affecting mRNA stability. Independently of its methyltransferase activity, CARM1 promotes PARP1 recruitment to replication forks, slowing fork progression (PubMed:33412112).

Q86X55

Observed band size: 63 kDa

affinity chromatography.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse

WB: 1:200-1:500

WB, IP

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant human PRMT4/CARM1 protein fragments expressed in E.coli.