Carbonic Anhydrase 2 Antibody (YA3362)

Carbonic Anhydrase 2 Antibody (YA3362) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3362), targeting Carbonic Anhydrase 2, with a predicted molecular weight of 29 kDa (observed band size: 29 kDa). Carbonic Anhydrase 2 Antibody (YA3362) can be used for WB, IHC-P experiment in human, mouse, rat background.

Carbonic Anhydrase 2 Protein is a member of the carbonic anhydrase isozyme family, responsible for catalyzing the reversible hydration of carbon dioxide. Dysregulation of this enzyme is linked to conditions such as osteopetrosis and renal tubular acidosis. Two transcript variants encoding distinct isoforms have been identified. In addition to its fundamental role in carbon dioxide metabolism, the protein exhibits biased expression in various tissues, with notable levels in the stomach and colon, as well as eight other tissues. This tissue-specific expression profile suggests its potential involvement in specialized physiological processes beyond its well-established functions in acid-base balance.

P00918

Predicted band size: 29 kDa; Observed band size: 29 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3105724

Cardiovascular

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1/500-1/1000;IHC: 1/50-1/100

• 
  Western blot analysis was performed on extracts from A431 (lane 1, 20 μg), Mouse kidney (lane 2, 20 μg), and Rat kidney (lane 3, 20 μg) using Carbonic Anhydrase 2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human Carbonic anhydrase 2