c-Kit Antibody (YA5533)

c-Kit Antibody (YA5533) is a mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to c-Kit/CD117. It can be applicated for IHC-P, WB, ICC/IF, ELISA assays, in the background of human.

c-Kit is a tyrosine-protein kinase that functions as a cell-surface receptor for the cytokine KITLG/SCF, playing a vital role in regulating cell survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development/function, and melanogenesis. Upon KITLG/SCF binding, c-Kit activates multiple signaling pathways, phosphorylating PIK3R1, PLCG1, SH2B2/APS, and CBL. It triggers the AKT1 pathway via PIK3R1 phosphorylation (the regulatory subunit of phosphatidylinositol 3-kinase) and transmits signals through GRB2 to activate RAS, RAF1, and MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. c-Kit also promotes STAT family member (STAT1/3/5A/5B) activation and PLCG1-mediated production of diacylglycerol and inositol 1,4,5-trisphosphate. Its signaling is modulated by protein phosphatases and receptor internalization/degradation, while activated c-Kit induces phosphorylation of PTPN6/SHP-1, PTPRU, STAT1/3/5A/5B, PIK3R1, CBL, CRK (Crk-II isoform), LYN, MAPK1/ERK2/ERK1, PLCG1, SRC, and SHC1.

P10721

Predicted band size: 120 kDa; Observed band size: 150 kDa

Protein G

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

IHC: 1:200-1000; WB: 1:500-2000; ICC/IF: 1:100-500; ELISA: 1:1000-5000

IHC-P, WB, ICC/IF, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Synthesized peptide derived from human CD117 AA range: 1-100