c-Fos Antibody (YA506)

c-Fos Antibody (YA506) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to c-Fos.

c-Fos is a nuclear phosphoprotein that forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, the basic regions of FOS and JUN/AP-1 each interact with symmetrical DNA half-sites. Upon TGF-beta activation, it forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. It plays a critical role in regulating the development of cells destined to form and maintain the skeleton and is thought to be important in signal transduction, cell proliferation, and differentiation. In growing cells, c-Fos activates phospholipid synthesis, likely by activating CDS1 and PI4K2A, an activity that requires Tyr dephosphorylation and association with the endoplasmic reticulum.

Free

P01100

Predicted band size: 41 kDa; Observed band size: 41/55 kDa

Protein A affinity purified.

Nucleus, Endoplasmic reticulum, Cytoplasm.

Non-conjugated

Unmodified

AB_3102070

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat

WB: 1:500 ;IHC-P: 1:50-1:200 ;ICC/IF: 1:100

• 
  Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using c-Fos antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
• 
  Immunohistochemical analysis of paraffin-embedded human ‌Cervical Carcinoma tissue using c-Fos antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
• 
  Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using c-Fos antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
• 
  Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 2) tissue using c-Fos antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

WB, IHC-P, ICC/IF

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human c-Fos.AA range:231-268.