Brk/PTK6 Antibody (YA1203)

Brk/PTK6 Antibody (YA1203) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1203), targeting Brk/PTK6. Brk/PTK6 Antibody (YA1203) can be used for IHC-P experiment in human background.

Brk Protein, a non-receptor tyrosine-protein kinase, intricately regulates diverse signaling pathways pivotal for the differentiation and maintenance of normal epithelia, as well as tumor growth. Its function appears to be context-dependent and varies based on cell type and intracellular localization. Brk Protein phosphorylates various substrates, including RNA-binding proteins (KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2, and SFPQ/PSF), transcription factors (STAT3 and STAT5A/B), and signaling molecules (ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS). It also associates with upstream proteins in signaling pathways, such as ADAM15, EGFR, ERBB2, ERBB3, and IRS4. In normal tissues, Brk Protein promotes cellular differentiation and apoptosis, while in tumors, it contributes to cancer progression by sensitizing cells to mitogenic signals, enhancing proliferation, anchorage-independent survival, and migration/invasion. Its association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development via augmentation of EGF-induced signaling through BTK/AKT and PI3 kinase. Brk Protein facilitates migration and proliferation by promoting EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP and activating RAS, while inactivating RhoA. Additionally, Brk Protein activates STAT3 and STAT5B to promote proliferation. The nuclear and cytoplasmic localization of Brk Protein plays distinct roles, with nuclear Brk Protein potentially regulating growth in normal epithelia, and cytoplasmic Brk Protein activating oncogenic signaling pathways.

Q13882

Predicted band size: 52 kDa; Observed band size: 52 kDa

Affinity Purified

Non-conjugated

Unmodified

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

IHC: 1/100-1/200

IHC-P

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.283.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Recombinant protein of human Brk