BRD2 Antibody (YA3910)

BRD2 Antibody (YA3910) is a mouse-derived and non-conjugated IgG2b antibody, targeting to BRD2. It can be applicated for WB, FC, ELISA assays, in the background of human, rat.

BRD2 is a chromatin reader protein that specifically recognizes and binds histone H4 acetylated at Lys-5 and Lys-12 (H4K5ac and H4K12ac, respectively), thereby regulating gene expression and remodeling chromatin structures. It recruits transcription factors and coactivators to target gene sites and activates RNA polymerase II machinery for transcriptional elongation. Additionally, BRD2 plays a key role in genome compartmentalization by associating with CTCF and cohesin: recruited to chromatin by CTCF, it promotes the formation of topologically associating domains (TADs) through its ability to bind acetylated histones, contributing to CTCF boundary formation and enhancer insulation. BRD2 also recognizes and binds acetylated non-histone proteins, such as STAT3, and is involved in inflammatory responses by regulating the differentiation of naive CD4(+) T-cells into Th17 cells. Notably, beyond acetylated lysines, BRD2 binds lysine residues on histones that are both methylated and acetylated (forming N6-acetyl-N6-methyllysine, Kacme), an epigenetic mark of active chromatin associated with increased transcriptional initiation.

P25440

Predicted band size: 88 kDa; Observed band size: 110 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Rat

WB: 1/500-1/2000; FC: 1/200-1/400; ELISA: 1/10000

WB, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Purified recombinant fragment of human BRD2 (AA: 227-364) expressed in E. Coli.