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  4. Monoclonal Antibodies Recombinant Antibodies
  5. BRCC36 Antibody (YA2298)

BRCC36 Antibody (YA2298)

Cat. No.: HY-P82553
User Guide for Antibodies Technical Support

BRCC36 Antibody (YA2298) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2298), targeting BRCC36, with a predicted molecular weight of 36 kDa (observed band size: 36 kDa). BRCC36 Antibody (YA2298) can be used for WB, IP experiment in human, mouse, rat background.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
50 μL Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
100 μL Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
250 μL   Get quote  
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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

BRCC36 Antibody (YA2298) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2298), targeting BRCC36, with a predicted molecular weight of 36 kDa (observed band size: 36 kDa). BRCC36 Antibody (YA2298) can be used for WB, IP experiment in human, mouse, rat background.
Background

BRCC36, identified as a metalloprotease, exhibits specificity in cleaving 'Lys-63'-linked polyubiquitin chains while displaying no activity toward 'Lys-48'-linked polyubiquitin chains. As a crucial component of the BRCA1-A complex, BRCC36 participates in DNA damage response by recognizing 'Lys-63'-linked ubiquitinated histones H2A and H2AX at sites of double-strand breaks. Within this complex, BRCC36 selectively removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, opposing the RNF8-dependent ubiquitination at double-strand breaks. Additionally, BRCC36 serves as the catalytic subunit of the BRISC complex, another multiprotein assembly dedicated to cleaving 'Lys-63'-linked ubiquitin in various substrates. The BRISC complex's involvement in mitotic spindle assembly and microtubule attachment to kinetochores, mediated by the deubiquitination of NUMA1, underscores its regulatory role in cell division. BRCC36 also contributes to interferon signaling through deubiquitination of the interferon receptor IFNAR1, enhancing its stability and cell surface expression. Furthermore, BRCC36 plays a role in regulating the NLRP3 inflammasome by mediating deubiquitination of NLRP3, leading to inflammasome assembly, and down-regulates the response to bacterial lipopolysaccharide by participating in IFNAR1 deubiquitination. In its repertoire, BRCC36 deubiquitinates HDAC1 and PWWP2B, contributing to their stabilization.
Gene ID
SwissProt ID
Synonyms
C6.1A; BRCC36; CXorf53
Molecular Weight

Predicted band size: 36 kDa; Observed band size: 36 kDa
Purity

Affinity Purified
Conjugation

Non-conjugated
Modification

Unmodified
Research Field

Cell Biology
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse, Rat
Dilution Ratio

WB: 1/500-1/1000;IP: 1/20
Application

WB, IP
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

A synthetic peptide of human BRCC36
Database

Entrez Gene: 79184 Human ; 210766 Mouse ; 316794 Rat

SwissProt: P46736 Human ; P46737 Mouse ; B2RYM5 Rat

OMIM: 300617 Human

Documentation
User Guide for Antibodies (1077 KB)

BRCC36 Antibody (YA2298) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BRCC36 Antibody (YA2298)
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