BPTF Antibody (YA4757)

BPTF Antibody (YA4757) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to BPTF.

BPTF protein functions as a regulatory subunit within the ATP-dependent NURF-1 and NURF-5 ISWI chromatin remodeling complexes, orchestrating the assembly of ordered nucleosome arrays on chromatin to facilitate DNA access during essential processes such as DNA replication, transcription, and repair. The NURF-1 ISWI chromatin remodeling complex, characterized by a lower ATP hydrolysis rate compared to NURF-5, plays a pivotal role in brain development by binding to the promoters of En1 and En2, positively regulating their expression. BPTF, as a histone-binding protein, exhibits a preference for H3 tails trimethylated on 'Lys-4' (H3K4me3), a marker for transcription start sites of active genes, and interacts with dimethylated H3 tails to a lesser extent. Beyond histone binding, BPTF may directly regulate transcription through interaction with DNA or transcription factors. It interacts with MAZ and KEAP1 and is a crucial component of the NURF-1 ISWI chromatin remodeling complex, which includes SMARCA1, BPTF, RBBP4, and RBBP7. Additionally, BPTF forms the catalytically inactive BPFT-SMARCA1 complex and is a component of the NURF-5 ISWI chromatin remodeling complex with SMARCA5/SNF2H. These interactions underscore the multifaceted role of BPTF in chromatin dynamics and transcriptional regulation.

Q12830

Predicted band size: 338 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:2000; ELISA: 1:10000

WB, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of human BPTF expressed in E. Coli.