BMP-2 Antibody

BMP-2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to BMP-2.

BMP2 is a growth factor of the TGF-beta superfamily that plays essential roles in various developmental processes, including cardiogenesis, neurogenesis, and osteogenesis. It induces cartilage and bone formation and initiates the canonical BMP signaling cascade by binding to type I receptor BMPR1A and type II receptor BMPR2. Upon complex formation at the cell surface, BMPR2 phosphorylates and activates BMPR1A, which in turn phosphorylates SMAD1/5/8 to regulate target gene transcription. BMP2 also promotes HAMP expression via interaction with BMPR1A/ALK3. Additionally, it signals through non-canonical pathways like the ERK/MAP kinase cascade to regulate osteoblast differentiation and stimulates myoblast-to-osteoblast differentiation via the EIF2AK3-EIF2A-ATF4 pathway. BMP2 acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, but its expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (by similar).

P12643

Predicted band size: 45 kDa; Observed band size: 60 kDa

affinity purified

Secreted.

Non-conjugated

Unmodified

AB_3103204

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

Chicken, Dog, Cow, Horse, Rabbit, Sheep

WB: 1:500-2000; ELISA: 1:5000-10000; IHC-P: 1:100-500; IHC-F: 1:100-500; IF: 1:100-500

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  Western blot analysis of extracts from M-Spleen using BMP2 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ELISA, IHC-P, IHC-F, ICC/IF

Liquid

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from human BMP2: 283-365/396