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  5. Bmi-1 Antibody (YA821)

Bmi-1 Antibody (YA821) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Bmi-1.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

Publications Citing Use of MCE Bmi-1 Antibody (YA821)

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Bmi-1 Antibody (YA821) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Bmi-1.
Background

BMI1 protein functions as a key component of a Polycomb group (PcG) multiprotein PRC1-like complex, crucial for maintaining the transcriptionally repressive state of various genes, including Hox genes, throughout development. This PcG PRC1 complex operates through chromatin remodeling and histone modification, specifically mediating monoubiquitination of histone H2A at 'Lys-119' to impart heritable changes in chromatin expressibility. Within the PRC1-like complex, BMI1 regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2. Interacting with various partners such as RING1, CBX7, CBX8, and PHC2, BMI1 is a critical player in nucleosomal binding and modification of histone H2A. Additionally, BMI1 forms part of a larger complex containing CUL3 and SPOP and interacts with E4F1 and zinc finger protein ZNF277, suggesting its involvement in intricate protein networks that contribute to chromatin dynamics and gene regulation during development.
Tag

Free
Gene ID
SwissProt ID
Molecular Weight

Predicted band size: 37 kDa; Observed band size: 40 kDa
Purity

Protein A affinity purified.
Subcellular Location

Nucleus, Cytoplasm
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Cell Biology

Product Categories

Primary Antibody; Mouse Monoclonal Antibody
Clonality

Monoclonal
Host

Mouse
Reactivity

Human, Mouse, Rat
Dilution Ratio

WB: 1:1000; IHC-P: 1:200; ICC: 1:50; FC: 1:100-1:200

  • Western blot analysis of extracts from K562 (lane 1) and Hela (lane 2) and Raji (lane 3) using Bmil antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-mouse IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Application

WB, ICC/IF, IHC-P, FC
Appearance

Liquid
Formulation

Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG2a
Sensitivity

Endogenous
Immunogen

Synthetic peptide corresponding to Human Bmi1.AA range: full length.
Database

Entrez Gene: 100532731 Human ; 648 Human ; 12151 Mouse ; 307151 Rat

SwissProt: P35226 Human ; P25916 Mouse ;

OMIM: 164831 Human

Documentation
SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

Bmi-1 Antibody (YA821) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Bmi-1 Antibody (YA821)
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HY-P80034
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