BMAL1 Antibody (YA4751)

BMAL1 Antibody (YA4751) is a mouse-derived and non-conjugated monoclonal antibody, targeting to BMAL1. It can be applicated for WB, IHC-P, ICC/IF, ELISA assays, in the background of human.

BMAL1 is a transcriptional activator that serves as a core component of the circadian clock, an internal timekeeping system generating approximately 24-hour rhythms in gene expression to regulate metabolism and behavior. Derived from the Latin 'circa' (about) and 'diem' (day), it governs diverse physiological processes including sleep, body temperature, blood pressure, and endocrine/immune functions. The clock consists of a central pacemaker in the suprachiasmatic nucleus (SCN) and peripheral oscillators in tissues, synchronized by Zeitgebers like light. BMAL1 heterodimerizes with CLOCK or NPAS2 to form the positive limb of the transcription-translation feedback loop (TTFL), activating E-box (5'-CACGTG-3')-containing clock genes (e.g., PER1/2/3, CRY1/2) and metabolic genes. These repressors then inhibit BMAL1 activity, completing the negative feedback. BMAL1 also regulates myogenesis (via Wnt signaling), pancreatic β-cell function (through NFE2L2-mediated antioxidant responses), and mTORC1 pathway suppression. It controls monocyte recruitment rhythms, ovarian steroidogenesis (e.g., CYP19A1), and hippocampal neurogenesis by modulating neural stem cell cycle entry. The CLOCK-BMAL1 heterodimer preferentially binds 5'-CACGTGA-3' and non-canonical E-box variants (e.g., 5'-AACGTGA-3'), driving rhythmic chromatin opening. In infection contexts, BMAL1 may post-transcriptionally regulate ACE2 and interferon-stimulated genes to influence SARS-CoV-2 entry. By similarity, it limits CD274 expression in macrophages (via PKM2) to combat sepsis and promotes skeletal muscle metabolic efficiency by activating DGAT2 and COQ10B.

O00327

Predicted band size: 69 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:2000; IHC: 1:200-1:1000; ELISA: 1:10000; ICC/IF: 1:50-200

WB, IHC-P, ICC/IF, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of human BMAL1 expressed in E. Coli.