beta Amyloid 1-42 Antibody (YA3555)

beta Amyloid 1-42 Antibody (YA3555) is an unconjugated, approximately 4 kDa, rabbit-derived, anti-beta Amyloid 1-42 monoclonal antibody.beta Amyloid 1-42 Antibody (YA3555) can be used for: WB, IHC-P, IF-Tissue expriments in human, mouse background without labeling.

APP functions as a cell surface receptor on neurons, playing roles in neurite growth, neuronal adhesion, and axonogenesis. Interactions between APP molecules on adjacent cells promote synaptogenesis. It participates in cell mobility and transcription regulation via protein-protein interactions, activating transcription through binding to APBB1-KAT5 while inhibiting Notch signaling via interaction with Numb. It couples to apoptosis pathways (e.g., G(o)- and JIP-mediated) and inhibits G(o) alpha ATPase activity (by similarity). As a kinesin I membrane receptor, it mediates axonal transport of beta-secretase and presenilin 1 (by similarity) and facilitates anterograde cargo transport toward synapses. It contributes to copper homeostasis/oxidative stress by reducing copper ions; copper-metallated APP induces neuronal death directly or via Cu²⁺-mediated LDL oxidation in vitro. By binding extracellular matrix components (e.g., heparin, collagen I/IV), it regulates neurite outgrowth. Splice isoforms containing the BPTI domain exhibit protease inhibitor activity. APP triggers an AGER-dependent pathway involving p38 MAPK activation, leading to amyloid-beta peptide internalization and mitochondrial dysfunction in cortical neurons. It supplies Cu²⁺ ions to GPC1 for NO release and heparan sulfate degradation. Amyloid-beta peptides act as lipophilic metal chelators, reducing Cu²⁺/Fe(3+) to Cu(+)/Fe²⁺ and binding lipoproteins (e.g., apoE/J, HDL) to inhibit metal-catalyzed oxidation. They promote tau aggregation and TPK II-mediated phosphorylation, while interaction with overexpressed HADH2 causes oxidative stress and neurotoxicity. Appicans enhance neural cell adhesion to the extracellular matrix and may regulate neurite outgrowth. Gamma-CTF and caspase-cleaved peptides (e.g., C31) strongly potentiate neuronal apoptosis.

P05067

Predicted band size: 4 kDa;Observed band size: 4 kDa

affinity purified

Cell membrane, Membrane, Perikaryon, Cell projection, growth cone, Membrane, clathrin-coated pit, Early endosome, Cytoplasmic vesicle.

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal, Recombinant

Rabbit

Human, Mouse

WB: 1:1,000; IHC-P: 1:1,000; IF-Tissue: 1:50

WB, IHC-P, ICC/IF

APP/PS1, 6-month mouse of AD brain tissue.

Liquid

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Synthetic peptide within Human beta Amyloid 1-42 peptide.