2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies Loading Controls Antibodies
  5. Beta Actin Antibody(YA3459)

Beta Actin Antibody(YA3459)

Cat. No.: HY-P83730
COA
SDS
User Guide for Antibodies Technical Support

Beta Actin Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to Beta Actin. It can be used as a loading control antibody.

For research use only. We do not sell to patients.

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100 μL In-stock
1 mL In-stock
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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Description

Beta Actin Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to Beta Actin. It can be used as a loading control antibody.
Host

Rabbit
Clonality

Monoclonal, Recombinant
Molecular Weight
Predicted band size: 42 kDa;
Observed band size: 42 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human beta Actin
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:5000-1:10000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:200
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100-1:200
Appearance

Liquid
Formulation

Supplied in 1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Jurkat (lane 2, 15 μg), RAW264.7 (lane 3, 15 μg), 3T3 (lane 4, 15 μg), and C6 (lane 5, 15 μg) using beta Actin Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:20000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:80000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Research Field

Cell Biology
Synonyms
ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta
Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Beta Actin Antibody(YA3459) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Beta Actin Antibody(YA3459)
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