Bcr Antibody (YA2746)

Bcr Antibody (YA2746) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2746), targeting Bcr, with a predicted molecular weight of 143 kDa (observed band size: 160 kDa). Bcr Antibody (YA2746) can be used for WB, IHC-P, IP experiment in human, rat background.

Bcr is a protein with a unique structure that exhibits two opposing regulatory activities toward small GTP-binding proteins. Its C-terminus contains a GTPase-activating protein (GAP) domain, which stimulates GTP hydrolysis by RAC1, RAC2, and CDC42, accelerating their intrinsic GTP hydrolysis rate and downregulating the active GTP-bound form. The central Dbl homology (DH) domain functions as a guanine nucleotide exchange factor (GEF), promoting the conversion of CDC42, RHOA, and RAC1 from the GDP-bound to the GTP-bound state. The N-terminus possesses intrinsic kinase activity. Bcr serves as a key negative regulator of neuronal RAC1 activity (by similar). It also modulates macrophage functions, including CSF1-directed motility and phagocytosis, through RAC1 activity regulation. Additionally, Bcr acts as a major RHOA GEF in keratinocytes, playing a role in focal adhesion formation and keratinocyte differentiation.

P11274

Predicted band size: 143 kDa; Observed band size: 160 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3105119

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat

WB: 1/500-1/1000;IHC: 1/50-1/100;IP: 1/20

• 
  Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), and HeLa (lane 2, 20 μg) using Bcr Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human Bcr