BAP1 Antibody (YA2733)

BAP1 Antibody (YA2733) is a non-conjugated IgG antibody, targeting BAP1, with a predicted molecular weight of 80 kDa (observed band size: 95 kDa). BAP1 Antibody (YA2733) can be used for WB experiment in human background.

The BAP1 protein functions as a deubiquitinating enzyme with a critical role in chromatin regulation, mediating the deubiquitination of histone H2A and HCFC1. As a catalytic component of the PR-DUB complex, it specifically targets histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1), highlighting its involvement in chromatin dynamics. BAP1 does not deubiquitinate monoubiquitinated histone H2B. It further serves as a regulator of cell growth by mediating the deubiquitination of HCFC1 N-terminal and C-terminal chains, displaying specificity towards 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Intriguingly, the deubiquitination of HCFC1 by BAP1 does not lead to an increase in HCFC1 stability. BAP1 interferes with the BRCA1 and BARD1 heterodimer activity, inhibiting their ability to mediate ubiquitination and autoubiquitination, although it does not directly mediate deubiquitination of BRCA1 and BARD1. Moreover, BAP1 can undergo autodeubiquitination via intramolecular interactions, counteracting monoubiquitination at the nuclear localization signal (NLS) and thereby protecting it from cytoplasmic sequestration. This multifaceted functionality positions BAP1 as a tumor suppressor.

Q92560

Predicted band size: 80 kDa; Observed band size: 95 kDa

Affinity Purified

Non-conjugated

Unmodified

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000

WB

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Recombinant protein of human BAP1