ATP5A Antibody (YA2306)

ATP5A Antibody (YA2306) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATP5A.

ATP5F1A, a crucial component of the mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V), plays a pivotal role in ATP production from ADP by harnessing the proton gradient across the membrane generated by the respiratory chain's electron transport complexes. Comprising the catalytic core (F(1)) and the membrane proton channel (F(0)), F-type ATPases, like ATP5F1A, consist of multiple subunits with distinct functions. The alpha and beta subunits form the catalytic core in F(1), where rotation of the central stalk against surrounding alpha(3)beta(3) subunits facilitates ATP hydrolysis. Intriguingly, ATP5F1A binds the bacterial siderophore enterobactin, promoting the accumulation of enterobactin-derived iron ions within mitochondria. Additionally, ATP5F1A interacts with various proteins, such as ATPAF2, HRG, PLG, BLOC1S1, BCL2L1 isoform BCL-X(L), CLN5, PPT1, S100A1, and ABCB7, influencing diverse cellular processes ranging from metabolic efficiency and iron homeostasis to apoptotic regulation. Furthermore, ATP5F1A is a crucial component of the ATP synthase complex, highlighting its central role in cellular energy production. Understanding these intricate interactions sheds light on the multifaceted functions of ATP5F1A in cellular physiology.

P25705

Predicted band size: 60 kDa; Observed band size: 55 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3104662

Tags & Cell Markers

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; FC: 1:50-1:100

• 
  Immunohistochemical analysis of paraffin-embedded rat kidney tissue using ATP5F1A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded rat kidney tissue using ATP5F1A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, FC

Liquid

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human ATP5A1