ATG5 Antibody (YA4487)

ATG5 Antibody (YA4487) is a mouse-derived and non-conjugated IgG2b antibody, targeting to ATG5. It can be applicated for WB, ICC/IF, FC, ELISA assays, in the background of human.

ATG5, a key participant in autophagic vesicle formation, undergoes conjugation with ATG12 through a ubiquitin-like system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme. The resulting ATG12-ATG5 conjugate serves as an E3-like enzyme essential for lipidation of ATG8 family proteins and their association with vesicle membranes. Beyond its role in autophagy, ATG5 contributes to mitochondrial quality control post-oxidative damage, impacting cellular longevity. It plays a critical role in lymphocyte development, vital for the survival and proliferation of both B and T lymphocytes, and is necessary for optimal antigen processing and presentation for MHC II. Additionally, ATG5 is involved in maintaining axon morphology, normal adipocyte differentiation, and promoting primary ciliogenesis through autophagic pathways. Moreover, it may play a crucial role in apoptotic processes, occurring downstream of caspase activity, and participates in IFN-gamma-induced autophagic cell death through interaction with FADD.

Q9H1Y0

Predicted band size: 32 kDa; Observed band size: 55 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

IF: 1/100-1/500; FC: 1/200-1/400; ELISA: 1/10000

WB, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Purified recombinant fragment of human ATG5 (AA: 144-275) expressed in E. Coli.