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  5. ATF6 Antibody (YA831)

ATF6 Antibody (YA831) is a non-conjugated and Mouse origined monoclonal antibody about 75 kDa, targeting to ATF6. It can be used for WB,IHC-P,FC assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

Publications Citing Use of MCE ATF6 Antibody (YA831)

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

ATF6 Antibody (YA831) is a non-conjugated and Mouse origined monoclonal antibody about 75 kDa, targeting to ATF6. It can be used for WB,IHC-P,FC assays with tag free, in the background of Human, Mouse, Rat.
Tag

Free
Gene ID
SwissProt ID
Molecular Weight

Predicted band size: 75 kDa
Purity

Protein G affinity purified.
Subcellular Location

Endoplasmic reticulum. Nucleus.
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Epigenetics and Nuclear Signaling
Product Categories

Primary Antibody; Mouse Monoclonal Antibody
Clonality

Monoclonal
Host

Mouse
Reactivity

Human, Mouse, Rat
Dilution Ratio

WB: 1:500-1:5,000 ;IHC-P: 1:50-1:100 ;FC:1:50-1:100

  • Western blot analysis of extracts from Hela(lane 2(20μg) , jurkat(lane 3(20μg) and RAW264.7(lane 4(20ug) using ATF6 (HY-P80379 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Application

WB, IHC-P, FC
Appearance

Liquid
Formulation

Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG1
Sensitivity

Endogenous
Immunogen

Synthetic peptide corresponding to Human ATF6.AA range:1-670.
Database

Entrez Gene: 22926 Human ; 226641 Mouse ; 304962 Rat

SwissProt: P18850 Human ;

OMIM: 616517 Human

Documentation

COA (191 KB)

User Guide for Antibodies (1077 KB)

ATF6 Antibody (YA831) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ATF6 Antibody (YA831)
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HY-P80379
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