ATF-2 Antibody (YA4740)

ATF-2 Antibody (YA4740) is a mouse-derived and non-conjugated monoclonal antibody, targeting to ATF-2. It can be applicated for WB, IHC-P, ICC/IF, ELISA assays, in the background of human, mouse.

ATF-2 is a transcriptional activator that regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Depending on its binding partner, ATF-2 binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, ATF-2 contributes to global transcription and the DNA damage response, as well as specific transcriptional activities related to cell development, proliferation, and death. In the cytoplasm, ATF-2 interacts with and disrupts HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, impairing mitochondrial membrane potential, inducing mitochondrial leakage, and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response, participating in ionizing radiation (IR)-induced S phase checkpoint control and the recruitment of the MRN complex to IR-induced foci (IRIF). ATF-2 exhibits histone acetyltransferase (HAT) activity, specifically acetylating histones H2B and H4 in vitro. In concert with CUL3 and RBX1, ATF-2 promotes the degradation of KAT5, thereby attenuating its ability to acetylate and activate ATM. Notably, ATF-2 can exhibit either oncogenic or tumor suppressor activities depending on the tissue or cell type.

P15336

Predicted band size: 55 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse

WB: 1:500-1:2000; IHC: 1:200-1:1000; ELISA: 1:10000; ICC/IF: 1:50-200

WB, IHC-P, ICC/IF, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of human ATF-2 expressed in E. Coli.