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  5. Argonaute 2 Antibody (YA612)

Argonaute 2 Antibody (YA612)

Cat. No.: HY-P80441
User Guide for Antibodies Technical Support

Argonaute 2 Antibody (YA612) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Argonaute 2.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Argonaute 2 Antibody (YA612) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Argonaute 2.
Background

Argonaute 2 (AGO2) is essential for RNA-induced silencing complex (RISC)-mediated gene silencing (RNAi). As the core of the 'minimal RISC', it binds to small guide RNAs like microRNAs (miRNAs) or short interfering RNAs (siRNAs) to direct RISC to complementary mRNA targets. Silencing mechanisms depend on complementarity: perfect matches trigger AGO2's endonucleolytic mRNA cleavage, while partial matches induce translation inhibition (endonuclease-independent). AGO2 suppresses translation initiation by either binding the 7-methylguanosine cap to block eIF4-E recruitment or interacting with EIF6 to prevent ribosomal subunit assembly, leading to target mRNA accumulation in P-bodies for potential degradation. Under specific conditions (e.g., serum starvation), AGO2 can enhance translation of certain mRNAs (e.g., TNF-α). It also mediates transcriptional gene silencing (TGS) via antigene RNAs (agRNAs). (Microbial infection) During SARS-CoV-2 infection, AGO2 may associate with viral miRNA-like CoV2-miR-O7a to repress host mRNAs (e.g., BATF2), aiding immune evasion.
Tag

Free
Gene ID
SwissProt ID
Molecular Weight

Predicted band size: 97 kDa; Observed band size: 97 kDa
Purity

Protein A affinity purified.
Subcellular Location

Cytoplasm, Nucleus.
Conjugation

Non-conjugated
Modification

Unmodified
Research Field

Epigenetics and Nuclear Signaling
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse
Dilution Ratio

WB: 1:1,000-1:2,000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:4,000; FC: 1:50-1:100; IP: Use at an assay dependent concentration.
Application

WB, ICC/IF, IHC-P, FC, IP
Appearance

Liquid
Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

Synthetic peptide corresponding to Human Argonaute 2.AA range:361-404.
Database

Entrez Gene: 27161 Human ; 239528 Mouse ; 59117 Rat

SwissProt: Q9UKV8 Human ; Q8CJG0 Mouse ; Q9QZ81 Rat

OMIM: 619149 Human

Documentation
User Guide for Antibodies (1077 KB)

Argonaute 2 Antibody (YA612) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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