APE1 Antibody (YA3923)

APE1 Antibody (YA3923) is a mouse-derived and non-conjugated IgG1 antibody, targeting to APEX1. It can be applicated for WB, IHC-P, FC, ELISA assays, in the background of human, rat, monkey.

APE1 is a multifunctional protein that plays a central role in the cellular response to oxidative stress. Its major activities include DNA repair and redox regulation of transcriptional factors. Functioning as an apurinic/apyrimidinic (AP) endodeoxyribonuclease in the base excision repair (BER) pathway, APE1 initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone, generating single-strand breaks with 5'-deoxyribose phosphate and 3'-hydroxyl ends. It also cleaves AP sites in DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. At switch sites of immunoglobulin (Ig) constant regions, APE1 mediates Ig isotype class switch recombination (By similarity). APE1 exhibits 3'-5' exodeoxyribonuclease activity on mismatched deoxyribonucleotides during short-patch BER. Additionally, it acts as an endoribonuclease involved in single-stranded RNA metabolism regulation, such as controlling MYC mRNA turnover by cleaving between UA and CA dinucleotides in the MYC coding region determinant (CRD). In association with NMD1, APE1 participates in the rRNA quality control process during cell cycle progression.

P27695

Predicted band size: 36 kDa; Observed band size: 36 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Rat, Monkey

WB: 1/500-1/2000; IHC: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human APEX1 (AA: 219-318) expressed in E. Coli.