AP2M1 Antibody (YA4418)

AP2M1 Antibody (YA4418) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to AP2M1.

AP2M1 is a component of the adaptor protein complex 2 (AP-2). AP-2 functions in protein transport via transport vesicles across different membrane traffic pathways, acting as vesicle coat components involved in cargo selection and vesicle formation. AP-2 participates in clathrin-dependent endocytosis, incorporating cargo proteins into clathrin-coated vesicles (CCVs) destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but cannot directly bind membrane components, whereas AP complexes interact with both the clathrin lattice and membrane lipids/proteins, serving as major clathrin adaptors for CCV formation. AP-2 also acts as a cargo receptor to selectively sort membrane proteins in receptor-mediated endocytosis and plays a role in recycling synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs (by similarity) and may regulate post-endocytic trafficking via the ARF6-dependent non-clathrin pathway. During hippocampal long-term potentiation, AP-2 mediates ADAM10 endocytosis. The AP-2 mu subunit binds transmembrane cargo proteins, recognizing Y-X-X-Phi motifs (by similarity), which become accessible upon Thr-156 phosphorylation in membrane-associated AP-2. This phosphorylation event involves assembled clathrin, which activates the AP-2 mu kinase AAK1. AP2M1 also contributes to Wnt-induced endocytosis of frizzled family members (by similarity).

Q96CW1

Predicted band size: 50 kDa; Observed band size: 50 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Monkey

WB: 1:300-1:800; ELISA: 1:10000

WB, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human AP2M1 (AA: 298-435) expressed in E. Coli.