2. Antibodies
  3. Secondary Antibodies
  4. Anti-Mouse lgG for IP (HRP)

Anti-Mouse lgG for IP (HRP)

Cat. No.: HY-P87497
COA
SDS
User Guide for Antibodies Technical Support

Anti-Mouse lgG for IP (HRP) is a conformation-specific secondary antibody that binds exclusively to native, non-denatured IgG and does not recognize denatured IgG. Conformation-specific secondary antibodies target spatial epitopes. When antibodies are denatured by high temperatures, they lose their native conformation and are no longer recognized by such antibodies—neither the heavy chain nor the light chain is detected. This effectively avoids interference from the heavy chain (55 kDa) and light chain (25 kDa) in immunoprecipitation (IP) experiments.

For research use only. We do not sell to patients.

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50 μL In-stock
100 μL In-stock
250 μL   Get quote  
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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Anti-Mouse lgG for IP (HRP) is a conformation-specific secondary antibody that binds exclusively to native, non-denatured IgG and does not recognize denatured IgG. Conformation-specific secondary antibodies target spatial epitopes. When antibodies are denatured by high temperatures, they lose their native conformation and are no longer recognized by such antibodies—neither the heavy chain nor the light chain is detected. This effectively avoids interference from the heavy chain (55 kDa) and light chain (25 kDa) in immunoprecipitation (IP) experiments.
Research Field

Immunology
Product Categories

Secondary Antibody
Clonality

Monoclonal
Host

Rat
Reactivity

Mouse
Dilution Ratio

WB: 1:2500-1:10000; ELISA: 1:1000-1:3000
Application

WB, ELISA
Appearance

Liquid
Formulation

Supplied in 1% BSA, 0.01% thimerosal, 50% glycerol, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year.Protect from light. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Immunogen

Mouse IgG
Documentation
SDS (251 KB)

COA (204 KB)

User Guide for Antibodies (1077 KB)

Anti-Mouse lgG for IP (HRP) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Anti-Mouse lgG for IP (HRP)
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