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  4. alpha Sarcoglycan Antibody (YA2068)

alpha Sarcoglycan Antibody (YA2068)

Cat. No.: HY-P82323
COA User Guide for Antibodies Technical Support

alpha Sarcoglycan Antibody (YA2068) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha Sarcoglycan.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

alpha Sarcoglycan Antibody (YA2068) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha Sarcoglycan.

Background

alpha Sarcoglycan is a component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex that forms a structural link between the F-actin cytoskeleton and the extracellular matrix.

Gene ID
SwissProt ID
Synonyms
SGCA; ADL; DAG2; Alpha-sarcoglycan; Alpha-SG; 50 kDa dystrophin-associated glycoprotein; 50DAG; Adhalin; Dystroglycan-2
Molecular Weight

Predicted band size: 43 kDa; Observed band size: 50 kDa

Purity

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Research Field

Signal Transduction

Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Clonality

Recombinant, Monoclonal

Host

Rabbit

Reactivity

Human, Mouse, Rat

Dilution Ratio

WB: 1:500-1:1000; IHC: 1:50-1:100; IP: 1:20

  • Western blot analysis was performed on extracts from Mouse skeletal muscle (lane 1, 15 μg), Rat skeletal muscle (lane 2, 15 μg), Mouse heart (lane 3, 15 μg), and Rat heart (lane 4, 15 μg) using alpha Sarcoglycan Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:90000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Application

WB, IHC-P, IP

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Sensitivity

Endogenous

Immunogen

A synthetic peptide of human alpha Sarcoglycan

Database
Documentation

alpha Sarcoglycan Antibody (YA2068) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
alpha Sarcoglycan Antibody (YA2068)
Cat. No.:
HY-P82323
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