AKT Antibody (YA5451)

AKT Antibody (YA5451) is a mouse-derived and non-conjugated IgG3 monoclonal antibody, targeting to AKT. It can be applicated for WB, IP, ICC/IF, ELISA assays, in the background of human, mouse, rat.

AKT1 Protein, a member of the AKT kinase family alongside AKT2 and AKT3, plays a pivotal role in regulating diverse cellular processes such as metabolism, proliferation, cell survival, growth, and angiogenesis. Functioning through serine and/or threonine phosphorylation of numerous downstream substrates, AKT1 is implicated in the modulation of over 100 reported substrate candidates, demonstrating its multifaceted nature. Notably, it orchestrates the insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter, impacting glucose uptake. Furthermore, AKT1 governs glucose storage as glycogen by phosphorylating GSK3A and GSK3B, inhibiting their kinase activity. Its involvement in various pathways includes mediating insulin-stimulated protein synthesis through the mTORC1 signaling pathway and regulating the FOXO factors, impacting cellular localization. AKT1 also influences NF-kappa-B-dependent gene transcription and positively regulates CREB1 activity, contributing to the transcription of pro-survival genes. Additionally, AKT1 exhibits a role in lipid metabolism by phosphorylating ATP citrate lyase (ACLY) and activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B), resulting in reduced cyclic AMP levels and inhibition of lipolysis. Beyond these functions, AKT1 is implicated in cell migration, adhesion assembly and disassembly, placental development, and diverse cellular responses to growth factors, including platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin, and insulin-like growth factor I (IGF-I). The recent discovery of its role in the SPATA13-mediated regulation of cell migration and adhesion adds to the complexity of its functions, highlighting AKT1's central role in cellular homeostasis and responsiveness to environmental cues.

P31749;P31751;Q9Y243

Observed band size: 60 kDa

Protein G

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat

WB: 1:500-2000; ICC/IF: 1:100-500; ELISA: 1:1000-5000

WB, IP, ICC/IF, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG3

Synthetic Peptide of human AKT at AA range of human 400-480 AA range: 370-477