2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. AER61 Antibody (YA2015)

AER61 Antibody (YA2015)

Cat. No.: HY-P82270
COA
SDS
User Guide for Antibodies Technical Support

AER61 Antibody (YA2015) is a non-conjugated IgG antibody, targeting AER61, with a predicted molecular weight of 62 kDa (observed band size: 62 kDa). AER61 Antibody (YA2015) can be used for WB experiment in human, rat background.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

AER61 Antibody (YA2015) is a non-conjugated IgG antibody, targeting AER61, with a predicted molecular weight of 62 kDa (observed band size: 62 kDa). AER61 Antibody (YA2015) can be used for WB experiment in human, rat background.
Background

AER61 is a glycosyltransferase that catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to serine or threonine residues in extracellular proteins, resulting in β-linked O-GlcNAc modification. This enzyme specifically glycosylates the threonine residue located between the fifth and sixth conserved cysteines of folded EGF-like domains (By similarity).
Gene ID
SwissProt ID
Synonyms
Extracellular O-linked N-acetylglucosamine transferase
Molecular Weight

Predicted band size: 62 kDa; Observed band size: 62 kDa
Purity

Affinity Purified
Conjugation

Non-conjugated
Modification

Unmodified
Research Field

Cell Biology
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Rat
Dilution Ratio

WB: 1/500-1/1000

  • Western blot analysis was performed on extracts from U-87MG (lane 1, 20 μg), HeLa (lane 2, 20 μg), Caco-2 (lane 3, 20 μg), C6 (lane 4, 20 μg), 293 (lane 5, 20 μg), and HepG2 (lane 6, 20 μg) using AER61 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Application

WB
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

Recombinant protein of human AER61
Database

Entrez Gene: 285203 Human ; 101351 Mouse ; 494219 Rat

SwissProt: Q5NDL2 Human ; Q8BYW9 Mouse ; Q5NDL0 Rat

OMIM: 615297 Human

Documentation
SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

AER61 Antibody (YA2015) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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AER61 Antibody (YA2015)
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